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Q. The basis of DNA finger printing is .........
A Knowledge of electrophoresis
B Knowledge of human karyotype
C Phenotypic difference in individuals
(D) Occurrence of restriction fragment length polymorphism

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Solution:
Genetic or DNA fingerprinting, DNA testing or DNA profiling is the technique used to distinguish between individuals of the same species using only samples of their DNA. It was invented by Sir Alec. Jeffreys. Variable Number of Tandem Repeat (VNTR) polymorphism is the basis of DNA fingerprinting which are short nucleotide repeats. The location and repetition in VNTR is so unique that no two individuals are alike. During DNA fingerprinting special DNA probes (single stranded labelled DNA rands) which with known sequences of probes complementary to these on VNTRs are used which bind to these VNTRs and make . them radiolabelled and thus observable. DNA fingerprinting may also use RFLP. DNA of each organism has specific sequences calle restriction fragments that can be cleaved by restriction endonuclease enzymes to produce fragments of different lengths. Occurrence of different lengths of cleaved DNA sequences is referred to as restriction fragment length polymorphism (RFLP).
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